Epithelial prostate cells RWPE1, PWR-1E, RWPE2 (a cell line that lacks p53 expression) and the primary tumor PCa cell line 22Rv1, were transfected with the ZEB1 coding sequence to analyze EMT markers and SDC-1 mRNA levels (Fig. 4a–d). Here, ZEB1 is linked to posterior cortical atrophy.