Importantly, IHC analysis of the tumors demonstrated that anti-Jag1 treatment imposed a significant increase in the extent of necrosis and fibrosis inside the tumor areas and a reduction in proliferation in the residual tumor mass as determined by Ki67 staining (Fig. 6e, f), which was not observed in the non-transformed adjacent colonic tissue (Fig. 6g). This evidence concerns the gene JAG1 and neoplasm.