JAG1 and adenoma: To experimentally test whether ectopic MFNG reverted JAG1 addiction in the adenoma cells, we transduced ApcMin/+;Jag1lox/lox;β-actin-Cre-ERT spheroids with retroviral control (pMIG) or a vector codifying for MFNG, and then treated the cultures with 5 μM 4-OH-tamoxifen to deplete adenoma cells from functional JAG1.