ApcMin/+ adenoma cells growing as three-dimensional (3D) structures displayed high protein levels of the ISC markers Lgr5, Bmi1, Cd44, and Ephb2, as well as Jag1 with rare cells expressing the intestinal differentiation markers Muc2, synaptophysin, CAII, and lysozyme (Supplementary Fig. 2B), in contrast to the non-transformed organoid structures (Supplementary Fig. 2C). This evidence concerns the gene EPHB2 and adenoma.