SOX9 and neoplasm: Supporting a role for Jag1 in adenoma formation and by extension in tumor-initiating cell (TIC) activity, Jag1 KO adenomas expressed reduced levels of Lgr5, Bmi1, Ephb2, c-Myc, Sox9, and Cd133 compared to the Jag1 WT, as determined by qRT-PCR (Fig. 2a) and in situ hybridization (ISH) (Fig. 2b, c).