While blocking only one of the two major phosphorylation sites of Akt (T308 or S473) still allowed the cells to undergo normal FOXO1-phosphorylation, genetic inhibition of both phosphorylation sites in Akt1-TASA overexpressing cells resulted in reduced FOXO1 phosphorylation and was associated with a strong inhibitory effect on the long-term survival of irradiated cancer cells. This evidence concerns the gene FOXO1 and cancer.