AKT1 and hepatocellular carcinoma: However, it remained controversial if cytoplasmic phosphorylation of Akt is dispensable for its nuclear access: Nguyen and colleagues showed that Akt1-TA and Akt1-SA failed to translocate to the nucleus in PC12 cells [36], whereas the double phosphorylation-deficient mutant Akt-TASA was found in the nuclear compartment using live imaging in hepatocellular carcinoma cells (HCC) [37].