To further determine the in vivo effect of DNMT1 on the increased stemness of OPN-over expressed HCC, CD133+/CD44+ cells with SCR/EV, CD133+/CD44+ cells with shOPN/EV and CD133+/CD44+ cells with shOPN/DNMT1 were subcutaneously implanted into NOD SCID mice. This evidence concerns the gene PROM1 and hepatocellular carcinoma.