However, TMAs are useful for examining large numbers of samples in standardized conditions and are widely used in studies that have examined PD‐L1 positivity in various cancers.10, 17, 35, 36, 37, 38, 39, 40 In addition, we systematically constructed the TMAs using large 2‐mm cores from the tumor's center and periphery and detected a significant correlation between PD‐L1 positivity at the two regions in the same tumor. This evidence concerns the gene CD274 and cancer.