The use of several biomarkers in ctDNA including the levels of overall ctDNA, ALU247 fragment concentration (26), KRAS mutations (27, 28), TP53 mutations (29, 30), BRAF mutations (28), and septin 9 (SEPT9) methylation (31–34) have been demonstrated for the diagnosis of CRC. The gene discussed is BRAF; the disease is colorectal carcinoma.