To understand whether GBPs and IRGB10 are involved in activation of caspase-11 during popB infection, Gbp2−/−, Gbp5−/−, Gbpchr3-KO and Irgb10−/− BMDMs were infected with popB. Interestingly, pyroptotic cell death, visualized by microscopy and quantified by Sytox green uptake was significantly reduced in both Gbpchr3-KO and Irgb10−/− BMDMs (Fig. 5a, Fig S3). The gene discussed is GBP2; the disease is infection.