Single-cell RNA-seq analysis of human melanoma tumor samplesFreshly resected samples were disaggregated to generate single-cell suspensions of mixed cells of unknown identitiesIndividual viable immune (CD45+) and nonimmune (CD45−) cells (including malignant and stromal cells) were recovered from the single-cell suspension by flow cytometrySingle cells were profiled by single-cell RNA-seq. The gene discussed is PTPRC; the disease is neoplasm.