Our results reproduced known differences: that HPV− HNSCC inactivates the cell cycle suppressor TP53 (7 HPV− vs. 1 HPV+ HNSCCs harbor variants) and CDKN2A (4 HPV− vs. 2 HPV+ HNSCCs harbor variants) by nonsynonymous single nucleotide polymorphism13. Here, TP53 is linked to head and neck squamous cell carcinoma.