Comparison between the time points of 48 and 72 h revealed significantly higher cells proliferation rate at 72 h (P<0.05), shown in Figure 2A. In order to explore and study the effect of HSG on the cell cycle and apoptosis of lung adenocarcinoma A549 cells, flow cytometry of PI staining and Annexin V-FITC double staining was conducted. Here, ANXA5 is linked to lung adenocarcinoma.