In the present study, HF diet inhibited the AMPKα phosphorylation and PPARα protein expression and accelerated the protein expressions of SREBP1c, SREBP2, and PPARγ, and increased the TC and TG synthesis in the livers, which can be exhibited by a significant reversed effect by both high- and low-MW chitosan supplementations. This evidence concerns the gene SREBF2 and hydrops fetalis.