To investigate whether or not the synergistic effects of GSPs and Res on the inhibition of cell viability and proliferation, as well as on the reduction of posttreatment colony forming ability in MDA-MB-231 and MCF-7 human breast cancer cells are associated with the induction of apoptosis, apoptosis analysis was performed by using the Annexin V-conjugated Alexafluor 488 (Alexa488) Apoptosis Vybrant Assay Kit, following the manufacture’s protocol. Here, ANXA5 is linked to breast cancer.