CD274 and neoplasm: The main findings of this study were that: Tumor mutation burden (TMB) is significantly associated with improved efficacy of combination immunotherapy: TMB was higher in patients with objective response compared with those with no response; computational predicted neoantigen burden correlates with response; TMB is independent of PD-L1 expression, but patients with high TMB and positive PD-L1 expression had significantly improved rates of objective responses and PFS, compared with those tumors with only one or neither variable [300].