Within ASNS, which has previously been associated with congenital microcephaly (Ruzzo et al., 2013; Gupta et al., 2017), two new mutations were identified: c.1165G > C, p.E389Q and c.601delA, p.M201Wfs∗28 (Table 2; all positions in the ASNS gene are annotated to RefSeq NM_133436.3, Ensembl ENST00000394309.3, GRCh37). The gene discussed is ASNS; the disease is Primary microcephaly.