Analyses of serum samples from the individuals with known outcomes showed that higher levels of IgG3 (Figure 5A) and a higher proportion of IgG3 (as %IgG; Figure 5B) in serum close to CIS diagnosis were inversely correlated with the number of days to conversion from CIS to MS (Figure 5). This evidence concerns the gene IGHG3 and in situ carcinoma.