In relapsed HGG patients, a DC-based vaccine preceded by one course of metronomic cyclophosphamide not only reduced Tregs but also led to a normalization of monocytic MDSC as well as pDC/cDC2 subsets and allowed induction of tumor-specific CD8+VLA-4+ T cells in > 90% of patients. Here, CD8A is linked to neoplasm.