After 24 h in this cancer-mimicking microenvironment, cells plated at high density showed a dramatic increase in the percentage of cells undergoing late stages of apoptosis (propidium iodide-positive) (Fig. 7a, b), as well as a large reduction in the cell viability (expressed as the percentage of cells double-negative for propidium iodide and annexin V)—Fig. 7c. Here, ANXA5 is linked to cancer.