The SSc fibrocytes treated for 3 h with various concentrations of CTLA4-Ig (10, 50, 100, and 500 μg/ml) did not show any significant variations in the gene expression levels of TGFβ, IL-1β, and CXCR2 compared with CNT (Fig. 2a). This evidence concerns the gene CTLA4 and systemic sclerosis.