To determine if CCL20 regulates breast cancer malignancy, firstly, we analyzed the database of The Cancer Genome Atlas (TCGA, https://cancergenome.nih.gov/) and found that CCL20 was highly expressed in TNBC in contrast to non-TNBC (Fig 3A), which was also observed at both mRNA and protein levels in breast cancer cell lines (Fig 3B and 3C). This evidence concerns the gene CCL20 and breast carcinoma.