To determine if CCL20 regulates breast cancer malignancy, firstly, we analyzed the database of The Cancer Genome Atlas (TCGA, https://cancergenome.nih.gov/) and found that CCL20 was highly expressed in TNBC in contrast to non-TNBC (Fig 3A), which was also observed at both mRNA and protein levels in breast cancer cell lines (Fig 3B and 3C). The gene discussed is CCL20; the disease is breast cancer.