MBNL1 and myotonic dystrophy type 2: In an effort to shed light onto the mechanisms of miR-1 misregulation, MBNL1 knocking down in cell culture blocked the maturation of pre-miR-1, which suggested a role of MBNL1 in miRNA processing and biogenesis, in agreement with the normal or elevated levels of pre-miR-1 found in DM1 and DM2 patients, respectively (113).