Interestingly, the PI3K/Akt pathway was also a highly likely candidate pathway to be targeted by either group of miRNAs (Figures 4A,B) The PI3K/Akt pathway is directly regulated by insulin signaling (Boucher et al., 2014) and has been shown to be dysregulated in skeletal muscle of people with T2DM (Krook et al., 1998, 2000); we therefore chose to examine which genes within this pathway would be targeted by the differentially expressed miRNAs. This evidence concerns the gene INS and type 2 diabetes mellitus.