MiR-409-3p has been known to target several genes such as MGMT, HMGN5 (glioblastoma)37,38, beclin, NLK and GAB1 (Colon cancer)39–41, AKT, ZEB1 (Breast cancer)42,43, c-Met (Lung cancer)44, radixin, PHF10 (gastric cancer)45,46. The gene discussed is NLK; the disease is breast cancer.