TWIST1 and neoplasm: Figure 3 shows the ROC curve for CTCs and Twist+ CTCs in predicting portal vein tumor thrombi, in which the assay demonstrated an area under the curve (AUC) of 0.872 and 0.927, respectively.  Figure 4 shows the ROC curve for CTCs and Twist+ CTCs in predicting tumor number, in which the assay demonstrated an AUC of 0.897 and 0.955, respectively.