To assess this and to investigate if there are molecular changes caused by the loss of TEM8, we created TEM8 KO clones of the human metastatic breast cancer cell line MDA-MB-231 (MDA) and the human metastatic colorectal cancer cell line SW620 using the CRISPR/Cas9 gene-editing technique. This evidence concerns the gene ANTXR1 and metastatic colorectal cancer.