To this end, H3F3A/B mutational testing using next generation sequencing (NGS) and Sanger DNA sequencing were comparatively performed with immunohistochemical stainings applying mutation-specific histone H3.3 antibodies, complemented with immunostainings against DOG1 as a marker of CB [20] and USP6-break apart fluorescent in situ hybridization (FISH) for the characterization of ABC. Here, ANO1 is linked to aneurysmal bone cyst.