To confirm that ART-EVs are present in the adult nervous system in vivo, we isolated the 100K EV fraction from human adult CSF (collected from a patient with glioblastoma multiforme) and purified SHH-containing vesicles, as well as CD63-positive exosomes from the 100K CSF pellet, by immunoprecipitation. Here, CD63 is linked to glioblastoma.