CD3E and infection: To determine whether TCRβ expression influenced the phagocytic function of macrophages, splenocytes labeled with macrophage (CD11b, CD14, and F4/80) and T cell (TCRβ and CD3ε) markers and CellTraceTM labeled peripheral pRBCs isolated from mice on day 3 post-infection were incubated in a 1:1 ratio for 90 minutes and the ability of TCRβ+ versus TCRβ− macrophages (Fig 10A) to phagocytose pRBCs was then compared by flow cytometry (Fig 10B).