As an alternative means of assessing the need for CD24 in Zika virus infection, both CD24 splice variants 1 and 7 were cloned from cDNA acquired from IMR-32 cells and sub-cloned into eukaryotic expression vectors (pcDNA6/CD24v1 and pcDNA5/CD24v7, respectively). Here, CD24 is linked to Zika virus infectious disease.