In the CCK8 assay, we found that the absorbance of the OTUB1‐isoform2‐overexpressing group was significantly higher than that of either the mock or the pcDNA3.1 control from 48 hours after transfection to the end of the measurement period in both A2780 and SKOV3 cells, suggesting that overexpression of OTUB1‐isoform2 elevated the accumulation of living ovarian tumour cells (P < .01, Figure 3A). The gene discussed is OTUB1; the disease is ovarian neoplasm.