Next, we verified the levels of autophagy in subcutaneous tumors by immunohistochemistry of p62 and LC3B-II (Fig. 5d), and the results showed the level of LC3B-II (indicated by the punctate staining of LC3B as the diffuse staining was LC3B-I) was significantly increased, while the expression of p62 was significantly reduced, suggesting that DIRAS3 overexpression may further inhibit subcutaneous tumor growth by inducing autophagy, compared with the vector-BGC-823 group. Here, MAP1LC3B is linked to neoplasm.