Compared with the vector-BGC-823 group (Fig. 5c), the tumor weight was significantly lower (3.63 ± 1.90 vs. 0.58 ± 0.70 g, P < 0.01) and the tumor volume was significantly smaller (4.83 ± 2.15 vs. 1.03 ± 1.25 cm3, P < 0.01) in the DIRAS3-BGC-823 group, indicating that DIRAS3 overexpression could inhibit the growth of subcutaneous tumor formed from BGC-823 gastric cancer cells in vivo. This evidence concerns the gene DIRAS3 and gastric cancer.