TFF2 and colitis: Therefore, we first induced colitis in wild-type mice (as well in Tff2-null mice) using 2.5% DSS in the drinking water for 5 days, followed on day 12 by the administration of Ad-Tff2 or Ad-Tff2-CTP-Flag, with additional mice treated with Ad-Fc or Ad-Fc-Flag as controls.