ARF6 and infection: To ensure that the enhanced ARF6-EEA1 association seen in infection was not merely a consequence of endocytic concentration at the VAC, we analyzed ARF6-EEA1 association in infected cells with endocytic pools ranging in size from ~160 μm2 to ~650 μm2 and found the ARF6-EEA1 association to be independent of the endocytic concentration (Fig. S2).