As part of a wider study, we designed a resequencing capture panel to the TWIST1 gene and flanking regions (2.4 Mb design with boundaries selected using human to mouse synteny; chr7:17346143‐19695462, GRCh38) and used this in the analysis of 14 SCS cases in whom no mutation of TWIST1 or other craniosynostosis‐associated genes had been identified (genetic screening was documented in all cases for TWIST1, and in the majority of cases for TCF12, FGFR2 exons IIIa and IIIc, and FGFR3 exon7 (Wilkie et al., 2017)). This evidence concerns the gene TWIST1 and craniosynostosis.