DNAJB1 and cyst: Interestingly, pVAX1-HSP40 immunization achieved a significant reduction in the parasite cyst burden in the brain of pVAX1-HSP40-immunized mice (1871.9 ± 142.3) compared with that of mice in the control groups immunized with pVAX1 (3479.2 ± 204.4), PBS (3024.4 ± 212.8), and healthy control mice (3275.0 ± 179.8), achieving 53.8%, 61.8%, and 57% reduction, respectively (p < 0.01; Fig. 4).