ACTB and cyst: The expression of biliary epithelial-related functions in the various cyst cultures from three independent experiments (n = 3) were measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analyses (Fig. 5) using Ck19, Mrp2, Asbt, Cftr, Sctr, and Mrp3 markers and β-actin as a housekeeping gene8,9,26–28 (Table 2).