To determine whether the cascade from increased concentrations of sphingomyelin in lysosomes and Golgi bodies and increased concentrations of ceramide in the ER to increased PP2A activity, the activation of Ulk, the phosphorylation and activation of Beclin, and thereby the stimulation of autophagy acts against MDD, we examined neurogenesis and behavior in unstressed and stressed mice treated with antidepressants or D609 in the presence or absence of spautin-1, an inhibitor of Beclin. This evidence concerns the gene PTPA and major depressive disorder.