AQP1 and malignant colon neoplasm: Western immunoblots demonstrated that unlike red blood cells (RBC) which had both monomeric (28 kDa) and glycosylated (30–40 kDa) forms (Supplementary Figure S2), the predominant form observed in colon cancer cell lines was the 56 kDa dimer, consistent with previous reports describing AQP1 in RBC, HT-29, SW480 and HCT116 [12,15,19].