One probe includes Q4 encapsulated organic nanoparticles (Q4NPs) for NIR-II imaging of tumor blood vessels, the other is composed of Q4 successfully conjugated with a small bombesin peptide to prepare the probe SCH1100 for gastrin-releasing peptide receptors (GRPR) targeted NIR-II imaging of prostate cancer in living mice. The gene discussed is GRPR; the disease is prostate carcinoma.