Total 18 fresh brain tissue specimens obtained from six patients (three tissues from each patient) with supratentorial, hemispheric, and diffuse high-grade gliomas (Table 1) were physically subdivided into control (n = 18), tumor infiltration (n = 18), and tumor mass (n = 18), which were confirmed by levels of GFAP and PSD95 in western blot (Figure 1D). This evidence concerns the gene DLG4 and neoplasm.