The inhibition of TBK1 binding to OPTN has been previously observed in ALS-linked TBK1 variants, mainly located in the C-terminal region of the protein (Freischmidt et al., 2015, Kim et al., 2016, Pozzi et al., 2017, Tsai et al., 2016). Here, TBK1 is linked to amyotrophic lateral sclerosis.