INS and Impaired glucose tolerance: As expected, the HFD group presented impaired glucose homeostasis with increased fasting blood glucose levels (151 ± 4 vs. 90 ± 2 mg/dL, p < 0.05) (Fig. 3A), elevated insulin levels (1.3 ± 0.2 vs. 0.3 ± 0.04 ng/mL, p < 0.05) (Fig. 3B), reduced insulin sensitivity (AUC: 9538 ± 440 vs. 13223 ± 449), p < 0.05) (Fig. 3C) and impaired glucose tolerance (AUC: 26361 ± 875.2 vs. 34354 ± 1358, p < 0.05)(Fig. 3D).