TNF and metabolic dysfunction-associated steatotic liver disease: The inflammatory state of the NAFLD was evaluated through: (1) western blot analysis of the TNF-α/JNK/Ikkβ/NFkB intracellular pathway, (2) the genetic expression of IL-1β, IL-10, F4-80 and arginase-1, and (3) the immunohistochemistry with an anti-F4-80 antibody in the liver of the obese mice.