In this study we observed that a limited number of ALS-linked miRNAs that are expressed in spinal motor neurons directly regulate NEFM and NEFH mRNA levels, in a way that might explain the increase in NFM and NFH protein levels that we observed in ALS spinal cords and thus contribute directly to the formation of NF NCIs. Here, NEFH is linked to amyotrophic lateral sclerosis.