To further confirm resibufogenin-induced RIP3-dependent cell death in colorectal carcinoma, HCT116 cells were stably transduced with lentivirus carrying RIP3 short hairpin RNA (shRNA) that display more than 80% reduced expression of RIP3 protein (Additional file 1, Additional file 2: Figure S15). The gene discussed is RIPK3; the disease is colorectal carcinoma.