Unprocessed COL1A1 in the media, representing the “lost collagen” (Fig. 2F, Supplementary Fig. S5) showed a reduced trend (DN = 2.19 ± 0.23, KC = 1.56 ± 0.35, measured by densitometric scans of the bands,) but did not reach significance (P = 0.18). The gene discussed is COL1A1; the disease is keratoconus.