Of note, consistent with our in vitro data, qRT‐PCR and immunoblot analysis revealed that the mRNA and protein expressions of CTSB were significantly reduced while E‐cad mRNA and protein levels were considerably increased in the tumor tissues derived from MTA1 knockdown (shMTA1) cells compared to NS controls (Fig. 5A–C). The gene discussed is CTSB; the disease is neoplasm.