When an osteosarcoma cell line (U2OS), a preosteoblastic cell line (MC3T3-E1), and an embryonic mesenchymal stem cell line (C3H/10T1/2) were exposed to NAM for 24 h, GAL4DBD-OSX transcriptional activity was dramatically decreased (Supplementary Fig. 3e). This evidence concerns the gene SP7 and osteosarcoma.