With a long term follow-up study, the B2M and CC16 (OR of 7.59 for developing ERFD) were found to be independent predictors for ERFD among T2D patients who had not yet manifested significant kidney disease at baseline and indicated that the protein peaks of B2M and CC16 detected by C18 plate/MALDI-TOF may improve the sensitivity for predicting nephropathy before the appearance of urinary albumin. The gene discussed is B2M; the disease is Nephropathy.