However, when including not only baseline samples but also follow-up samples, thereby increasing total n from 41 to 161, RRMS patients had higher CSF-NFL (p = 0.01), but not S-NFL (p = 0.6), than CIS patients, and NFL levels were higher in relapse patients than in non-relapse patients (p = 0.004 for S-NFL and p ≤ 0.001 for CSF-NFL). The gene discussed is NEFL; the disease is in situ carcinoma.