In neuroblastoma cells in which TRPM2 was depleted with CRISPR, both the abundance of phosphorylated Pyk2 (group × exposure time interaction effect, P = 0.0025) and Pyk2 expression (group × exposure time interaction effect, P < 0.0001) were significantly reduced with doxorubicin exposure (Fig. 1, B and C). This evidence concerns the gene PTK2B and neuroblastoma.