The detailed analyses of the 5′-end CpG island methylation status of TUSC3, ATRNL1, POMT1 and SAMD4A in a set of 50 additional human colorectal cancer cell lines beyond HCT-116, including their microsatellite instability status, is shown in Supplementary Figure 6. The gene discussed is SAMD4A; the disease is colorectal cancer.